Identification of Canine Calicivirus Capsid Protein and Its Immunoreactivity in Western Blotting.
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چکیده
منابع مشابه
Identification of canine calicivirus capsid protein and its immunoreactivity in western blotting.
A canine calicivirus (CaCV) isolated in Japan, designated as CaCV No. 48 strain, was propagated in MDCK cells and purified by CsCl equilibrium gradient centrifugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the purified samples revealed the presence of only one major species of viral protein of about 60 kilodaltons after Coomassie staining. The same band, presumab...
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This report describes the detailed procedures for Western blot analysis using fluorescent antibodies. After electrophoresis and subsequent electroblotting, the fluorescent-labeled antibodies were visible upon ultraviolet illumination of the polyvinylidene fluoride (PVDF) membranes and could then be photographed to give an accurate record of the blots. Fluorescent labeling allows for photographi...
متن کاملIdentification of conformational neutralizing epitopes on the capsid protein of canine calicivirus.
Two neutralizing monoclonal antibodies (MAbs) against canine calicivirus (CaCV), which has a distinct antigenicity from feline calicivirus (FCV), were obtained. Both MAbs recognized conformational epitopes on the capsid protein of CaCV and were used to identify these epitopes. Neutralization-resistant variants of CaCV were selected in the presence of individual MAbs in a cell culture. Cross-neu...
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منطقه مورد مطالعه در 23 کیلومتری جنوب غرب شهرستاشاهرود قرار دارد که در نقشه 100000/1 شاهرود قرار گرفته است.ناحیه مورد مطالعه در تقسیمات ساختاری ایران بخشی از زون البرز شرقی است . در طی سنوزوئیک این زون به شدت تحت تأثیر فازهای کوهزایی آلپی قرار گرفته و فعالیت های آتشفشانی انوسن در قسمت های غربی آن دیده می شود . از نظر ترکیب سنگ شناسی منطقه مورد مطالعه متنوع و بیشتر شامل سنگ های رسوبی مانند : آ...
15 صفحه اولAnalysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein.
Three isolates of feline calicivirus (FCV) designated NADC, KCD and CFI/68 were compared for biochemical, serological and genetic variation within the capsid protein gene. The M(r) of the capsid protein from purified virions was approximately 66,000 for the NADC virus isolate, which differed slightly from the relative mobilities of the purified capsid proteins of the KCD and CFI/68 isolates. Po...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 1997
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.59.97